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FAQ: Laminin coating technical questions and answers

 

As the leading manufacturer and supplier of laminin coated coverslips, neuVitro invented a 16-step, 5-day procedure with our state of the art facility to generate the best professional coating on German coverslips for challenged primary cell culture. All coated coverslips are sterilized by radiation and ready to use for immediate high-quality cell culture works.

 

5-day laminin coating procedure

1.       Wash 2 hours in hot dd water.

2.       Rinse x 6 times.

3.       Treat in 100% ethanol 2 hours.

4.       Treat in 70% Nitric acid 24 hours.

5.       Rinse 6 times x 1 hour each.

6.       Priming coating with PDL

7.       Rinse x 2 times.

8.       Laminin coating x 24 hours

9.       Rinse x 2 times

10.    Dry overnight

11.    Seal into bags, 15 pieces/bag

12.    Sterilization by radiation

Note:

This protocol is for automated coating machine only. Not suitable for lab.

 

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Neuvitro Corporation

The coverslip coating expert  

 

 

with G3 Digital Coverslip Coating System

 

 

Poly-D-lysine coating

Pre-coated coverslips

Ready to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell culture, starting $55/box

 

Rat tail Collagen

Pre-coated coverslips

Ready to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell culture, starting $65/box

 

Fibronectin coating

Pre-coated coverslips

Ready to use German coverslip with fibronectin coating on both sides for cell culture, starting $89/box

 

Laminin coating

Pre-coated coverslips

Ready to use German coverslip with human Laminin coating on both sides for cell culture and neuron culture, starting $94/box

 

Gelatin coating

Pre-coated coverslips

Ready to use German coverslip with porcine gelatin coating on both sides for cell culture, starting $89/box

 

German Coverslips 

Cell culture tested

CE certified, tested for primary cell and primary neuron cultures, starting $12/box

 

Liberty 120

High-speed gel system

See you PCR and genotyping results in 6 min with straight banding technology...