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  polylysine coating protocol

Get professional poly lysine coated coverslips

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      neuVitro      

 German coverslip with digital precise polylysine coating

 

Professional polylysine coating procedure for enhanced cell culture 

 

This protocol works very well for primary cell culture and primary neuron culture as shown at right

 

It can be adapted to coat culture dishes and plates.

 

Click to enlarge neuronal culture

Wash coverslips 2 hours on shaker.

Rinse coverslips 6 times x 30 minutes each on shaker.

Treat coverslips in 100% Ethanol x 2 hour at room temperature.

Treat coverslips in 70% concentrated Nitric Acid x 24 hours at room temperature.

Rinse coverslips in dd-water 6 times x 1 hour each on shaker.

Primary coating, 1mg/ml, 12 hours at room temperature.

Rinse coverslips x 2 times to remove unbound poly lysine

Dry coverslips 12 hours in laminar hood.

Secondary coating, 1mg/ml, 12 hours at room temperature.

Rinse coverslips x 2 times to remove unbound poly lysine

Dry coverslips 12 hours in laminar hood.

Seal coated coverslips into bags, 15 units/bag.

Sterilization by radiation.

Quality inspection.

Note:

This protocol is for automated coating machine only. Not suitable for lab.

 

Cell Culture Coverslips

 

  

German Coverslips 

Cell culture tested

CE certified, tested for primary cell and primary neuron cultures, starting $12/box

 

Poly-D-lysine coating

Pre-coated coverslips

Ready to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell culture, starting $55/box

 

Rat tail Collagen

Pre-coated coverslips

Ready to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell culture, starting $65/box

 

Fibronectin coating

Pre-coated coverslips

Ready to use German coverslip with fibronectin coating on both sides for cell culture, starting $89/box

 

Laminin coating

Pre-coated coverslips

Ready to use German coverslip with human Laminin coating on both sides for cell culture and neuron culture, starting $94/box

 

Gelatin coating

Pre-coated coverslips

Ready to use German coverslip with porcine gelatin coating on both sides for cell culture, starting $89/box

 

Liberty 120

High-speed gel system

See you PCR and genotyping results in 6 min with straight banding technology...