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One-step mouse genotyping DNA extraction protocol

See mouse genotyping PCR results in 6 minutes

One-step mouse genotyping DNA extraction kit -- No spin, No purification!

Using the fast genotyping kit, all you need to do is to heat the samples for 1 hour. That's it! 

 

In the genotyping protocol, DNA was extracted from mouse tails or toes using the kit with 1-hour heating at 94 degrees, then 1ul of the each heated sample was used directly for genotyping PCR without spin or purification. Negative controls (lane 9-11) received no mouse DNA.

 

Cat. # Item Description  Price Order online
GDE-250

Mouse genotyping DNA extraction kit, 250 applications

$38
BA-500 Highly purified agarose, molecular grade for DNA, RNA, PCR, genotyping gel electrophoresis, 500 gram bottle, is $219 per bottle during 40% discount promotion was $365     now    $219

     

Why agarose-saving feature is so important for genotyping and PCR applications?

In agarose electrophoresis, the gel concentration is determined by the size range of the DNA (or RNA) fragments being tested, with much higher percentage gels being used to resolve small sizes (100-200bp, for example). For  reliability, most PCR primers were designed to generate PCR products in the small size range, which means that your agarose gel should be made to 3% or higher. 

 

Liberty 120 gel system can save 60% agarose for you.

 

Neuvitro Corporation

The coverslip coating expert  

 

with G3 Digital Coverslip Coating System

 

 

Poly-D-lysine coating

Pre-coated coverslips

Ready to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell culture & confocal fluorescence microscopy, starting $55/box

 

Rat tail Collagen

Pre-coated coverslips

Ready to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell culture & confocal fluorescence microscopy, starting $65/box

 

Fibronectin coating

Pre-coated coverslips

Ready to use German coverslip with fibronectin coating on both sides for cell culture & confocal fluorescence microscopy, starting $89/box

 

Laminin coating

Pre-coated coverslips

Ready to use German coverslip with human Laminin coating on both sides for cell culture & confocal fluorescence microscopy, starting $94/box

 

BME coating

Pre-coated coverslips

Ready to use German coverslip with basement membrane extract (BME) coating on both sides for cell culture & confocal fluorescence microscopy, starting $89/box

 

Gelatin coating

Pre-coated coverslips

Ready to use German coverslip with porcine gelatin coating on both sides for cell culture & confocal fluorescence microscopy, starting $89/box

 

German Coverslips 

Cell culture tested

CE certified, tested for primary cell and primary neuron cultures, starting $12/box